mab 35 Search Results


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Developmental Studies Hybridoma Bank mab 35
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Covance anti-nicotinic acetylcholine receptor iggs mab clone 35
Caveolin-3 expression promotes activation of MuSK and Rac-1 after agrin treatment. (A) Ras G12V -transformed 3T3 cells were transiently cotransfected with caveolin-3 and each of the following constructs: pCMV-HA, pCMV-WT-MuSK-HA, pCMV-mutant A-MuSK-HA, and pCMV-mutant B-MuSK-HA. Forty eight hours after transfection, cell lysates were immunoprecipitated with an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting analysis using anti-HA <t>IgGs.</t> Total expression of Cav-3 and HA-tag–expressing constructs are shown in the two bottom panels. (B–D) Myogenic precursor cells were derived from wild-type and caveolin-3 null mice, differentiated for 7 d, and treated with agrin (10 ng/ml) for 15 min, 1 h, or 4 h. Untreated cells were used as controls. (B) Cell lysates were immunoprecipitated using an antibody probe specific for MuSK, and immunoprecipitates were subjected to immunoblotting analysis using either anti-phosphotyrosine (P-Tyr) or anti-caveolin-3 IgGs. Total MuSK, caveolin-3, and phosphorylated JNK expression are shown in the bottom panel. (C) Cell lysates were immunoprecipitated using GST fused to the PBD of Pak1, and immunoprecipitates were subjected to immunoblotting analysis using anti-Rac-1 IgGs. Total Rac-1 expression is shown in the bottom panel. (D) Cell lysates were immunoprecipitated using an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting using anti-Rac-1 IgGs. Total caveolin-3 and Rac-1 expression are shown in the bottom panel.
Anti Nicotinic Acetylcholine Receptor Iggs Mab Clone 35, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mimotopes mab cs-35
Caveolin-3 expression promotes activation of MuSK and Rac-1 after agrin treatment. (A) Ras G12V -transformed 3T3 cells were transiently cotransfected with caveolin-3 and each of the following constructs: pCMV-HA, pCMV-WT-MuSK-HA, pCMV-mutant A-MuSK-HA, and pCMV-mutant B-MuSK-HA. Forty eight hours after transfection, cell lysates were immunoprecipitated with an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting analysis using anti-HA <t>IgGs.</t> Total expression of Cav-3 and HA-tag–expressing constructs are shown in the two bottom panels. (B–D) Myogenic precursor cells were derived from wild-type and caveolin-3 null mice, differentiated for 7 d, and treated with agrin (10 ng/ml) for 15 min, 1 h, or 4 h. Untreated cells were used as controls. (B) Cell lysates were immunoprecipitated using an antibody probe specific for MuSK, and immunoprecipitates were subjected to immunoblotting analysis using either anti-phosphotyrosine (P-Tyr) or anti-caveolin-3 IgGs. Total MuSK, caveolin-3, and phosphorylated JNK expression are shown in the bottom panel. (C) Cell lysates were immunoprecipitated using GST fused to the PBD of Pak1, and immunoprecipitates were subjected to immunoblotting analysis using anti-Rac-1 IgGs. Total Rac-1 expression is shown in the bottom panel. (D) Cell lysates were immunoprecipitated using an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting using anti-Rac-1 IgGs. Total caveolin-3 and Rac-1 expression are shown in the bottom panel.
Mab Cs 35, supplied by Mimotopes, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sterogene mab 35
Caveolin-3 expression promotes activation of MuSK and Rac-1 after agrin treatment. (A) Ras G12V -transformed 3T3 cells were transiently cotransfected with caveolin-3 and each of the following constructs: pCMV-HA, pCMV-WT-MuSK-HA, pCMV-mutant A-MuSK-HA, and pCMV-mutant B-MuSK-HA. Forty eight hours after transfection, cell lysates were immunoprecipitated with an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting analysis using anti-HA <t>IgGs.</t> Total expression of Cav-3 and HA-tag–expressing constructs are shown in the two bottom panels. (B–D) Myogenic precursor cells were derived from wild-type and caveolin-3 null mice, differentiated for 7 d, and treated with agrin (10 ng/ml) for 15 min, 1 h, or 4 h. Untreated cells were used as controls. (B) Cell lysates were immunoprecipitated using an antibody probe specific for MuSK, and immunoprecipitates were subjected to immunoblotting analysis using either anti-phosphotyrosine (P-Tyr) or anti-caveolin-3 IgGs. Total MuSK, caveolin-3, and phosphorylated JNK expression are shown in the bottom panel. (C) Cell lysates were immunoprecipitated using GST fused to the PBD of Pak1, and immunoprecipitates were subjected to immunoblotting analysis using anti-Rac-1 IgGs. Total Rac-1 expression is shown in the bottom panel. (D) Cell lysates were immunoprecipitated using an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting using anti-Rac-1 IgGs. Total caveolin-3 and Rac-1 expression are shown in the bottom panel.
Mab 35, supplied by Sterogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson pkc -specific mab 27
Caveolin-3 expression promotes activation of MuSK and Rac-1 after agrin treatment. (A) Ras G12V -transformed 3T3 cells were transiently cotransfected with caveolin-3 and each of the following constructs: pCMV-HA, pCMV-WT-MuSK-HA, pCMV-mutant A-MuSK-HA, and pCMV-mutant B-MuSK-HA. Forty eight hours after transfection, cell lysates were immunoprecipitated with an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting analysis using anti-HA <t>IgGs.</t> Total expression of Cav-3 and HA-tag–expressing constructs are shown in the two bottom panels. (B–D) Myogenic precursor cells were derived from wild-type and caveolin-3 null mice, differentiated for 7 d, and treated with agrin (10 ng/ml) for 15 min, 1 h, or 4 h. Untreated cells were used as controls. (B) Cell lysates were immunoprecipitated using an antibody probe specific for MuSK, and immunoprecipitates were subjected to immunoblotting analysis using either anti-phosphotyrosine (P-Tyr) or anti-caveolin-3 IgGs. Total MuSK, caveolin-3, and phosphorylated JNK expression are shown in the bottom panel. (C) Cell lysates were immunoprecipitated using GST fused to the PBD of Pak1, and immunoprecipitates were subjected to immunoblotting analysis using anti-Rac-1 IgGs. Total Rac-1 expression is shown in the bottom panel. (D) Cell lysates were immunoprecipitated using an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting using anti-Rac-1 IgGs. Total caveolin-3 and Rac-1 expression are shown in the bottom panel.
Pkc Specific Mab 27, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Johns Hopkins HealthCare mab 35
Caveolin-3 expression promotes activation of MuSK and Rac-1 after agrin treatment. (A) Ras G12V -transformed 3T3 cells were transiently cotransfected with caveolin-3 and each of the following constructs: pCMV-HA, pCMV-WT-MuSK-HA, pCMV-mutant A-MuSK-HA, and pCMV-mutant B-MuSK-HA. Forty eight hours after transfection, cell lysates were immunoprecipitated with an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting analysis using anti-HA <t>IgGs.</t> Total expression of Cav-3 and HA-tag–expressing constructs are shown in the two bottom panels. (B–D) Myogenic precursor cells were derived from wild-type and caveolin-3 null mice, differentiated for 7 d, and treated with agrin (10 ng/ml) for 15 min, 1 h, or 4 h. Untreated cells were used as controls. (B) Cell lysates were immunoprecipitated using an antibody probe specific for MuSK, and immunoprecipitates were subjected to immunoblotting analysis using either anti-phosphotyrosine (P-Tyr) or anti-caveolin-3 IgGs. Total MuSK, caveolin-3, and phosphorylated JNK expression are shown in the bottom panel. (C) Cell lysates were immunoprecipitated using GST fused to the PBD of Pak1, and immunoprecipitates were subjected to immunoblotting analysis using anti-Rac-1 IgGs. Total Rac-1 expression is shown in the bottom panel. (D) Cell lysates were immunoprecipitated using an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting using anti-Rac-1 IgGs. Total caveolin-3 and Rac-1 expression are shown in the bottom panel.
Mab 35, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Unwin Ltd mab 35
Caveolin-3 expression promotes activation of MuSK and Rac-1 after agrin treatment. (A) Ras G12V -transformed 3T3 cells were transiently cotransfected with caveolin-3 and each of the following constructs: pCMV-HA, pCMV-WT-MuSK-HA, pCMV-mutant A-MuSK-HA, and pCMV-mutant B-MuSK-HA. Forty eight hours after transfection, cell lysates were immunoprecipitated with an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting analysis using anti-HA <t>IgGs.</t> Total expression of Cav-3 and HA-tag–expressing constructs are shown in the two bottom panels. (B–D) Myogenic precursor cells were derived from wild-type and caveolin-3 null mice, differentiated for 7 d, and treated with agrin (10 ng/ml) for 15 min, 1 h, or 4 h. Untreated cells were used as controls. (B) Cell lysates were immunoprecipitated using an antibody probe specific for MuSK, and immunoprecipitates were subjected to immunoblotting analysis using either anti-phosphotyrosine (P-Tyr) or anti-caveolin-3 IgGs. Total MuSK, caveolin-3, and phosphorylated JNK expression are shown in the bottom panel. (C) Cell lysates were immunoprecipitated using GST fused to the PBD of Pak1, and immunoprecipitates were subjected to immunoblotting analysis using anti-Rac-1 IgGs. Total Rac-1 expression is shown in the bottom panel. (D) Cell lysates were immunoprecipitated using an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting using anti-Rac-1 IgGs. Total caveolin-3 and Rac-1 expression are shown in the bottom panel.
Mab 35, supplied by Unwin Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Caveolin-3 expression promotes activation of MuSK and Rac-1 after agrin treatment. (A) Ras G12V -transformed 3T3 cells were transiently cotransfected with caveolin-3 and each of the following constructs: pCMV-HA, pCMV-WT-MuSK-HA, pCMV-mutant A-MuSK-HA, and pCMV-mutant B-MuSK-HA. Forty eight hours after transfection, cell lysates were immunoprecipitated with an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting analysis using anti-HA IgGs. Total expression of Cav-3 and HA-tag–expressing constructs are shown in the two bottom panels. (B–D) Myogenic precursor cells were derived from wild-type and caveolin-3 null mice, differentiated for 7 d, and treated with agrin (10 ng/ml) for 15 min, 1 h, or 4 h. Untreated cells were used as controls. (B) Cell lysates were immunoprecipitated using an antibody probe specific for MuSK, and immunoprecipitates were subjected to immunoblotting analysis using either anti-phosphotyrosine (P-Tyr) or anti-caveolin-3 IgGs. Total MuSK, caveolin-3, and phosphorylated JNK expression are shown in the bottom panel. (C) Cell lysates were immunoprecipitated using GST fused to the PBD of Pak1, and immunoprecipitates were subjected to immunoblotting analysis using anti-Rac-1 IgGs. Total Rac-1 expression is shown in the bottom panel. (D) Cell lysates were immunoprecipitated using an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting using anti-Rac-1 IgGs. Total caveolin-3 and Rac-1 expression are shown in the bottom panel.

Journal: Molecular Biology of the Cell

Article Title: Caveolin-3 Promotes Nicotinic Acetylcholine Receptor Clustering and Regulates Neuromuscular Junction Activity

doi: 10.1091/mbc.E09-05-0381

Figure Lengend Snippet: Caveolin-3 expression promotes activation of MuSK and Rac-1 after agrin treatment. (A) Ras G12V -transformed 3T3 cells were transiently cotransfected with caveolin-3 and each of the following constructs: pCMV-HA, pCMV-WT-MuSK-HA, pCMV-mutant A-MuSK-HA, and pCMV-mutant B-MuSK-HA. Forty eight hours after transfection, cell lysates were immunoprecipitated with an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting analysis using anti-HA IgGs. Total expression of Cav-3 and HA-tag–expressing constructs are shown in the two bottom panels. (B–D) Myogenic precursor cells were derived from wild-type and caveolin-3 null mice, differentiated for 7 d, and treated with agrin (10 ng/ml) for 15 min, 1 h, or 4 h. Untreated cells were used as controls. (B) Cell lysates were immunoprecipitated using an antibody probe specific for MuSK, and immunoprecipitates were subjected to immunoblotting analysis using either anti-phosphotyrosine (P-Tyr) or anti-caveolin-3 IgGs. Total MuSK, caveolin-3, and phosphorylated JNK expression are shown in the bottom panel. (C) Cell lysates were immunoprecipitated using GST fused to the PBD of Pak1, and immunoprecipitates were subjected to immunoblotting analysis using anti-Rac-1 IgGs. Total Rac-1 expression is shown in the bottom panel. (D) Cell lysates were immunoprecipitated using an antibody probe specific for caveolin-3, and immunoprecipitates were subjected to immunoblotting using anti-Rac-1 IgGs. Total caveolin-3 and Rac-1 expression are shown in the bottom panel.

Article Snippet: Anti-nicotinic acetylcholine receptor IgGs (mAb clone 35) and anti-β-tubulin 3 IgGs (mAb TUJ1) were from Covance (Berkeley, CA).

Techniques: Expressing, Activation Assay, Transformation Assay, Construct, Mutagenesis, Transfection, Immunoprecipitation, Western Blot, Derivative Assay